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FASTQ Interleaver

Interleave paired R1/R2 FASTQ files for transcript assembly.

Overview

Combine paired-end R1 and R2 FASTQ files into one interleaved FASTQ with strict record and pairing validation. Supports plain and gzip-compressed inputs.

Run FASTQ Interleaver on Neurosnap

The FASTQ Interleaver online webserver allows anybody with a Neurosnap account to run and access FASTQ Interleaver, no downloads required. Information submitted through this webserver is kept confidential and never sold to third parties as detailed by our strong Terms of Use and Privacy Policy.

FASTQ Interleaver service preview

Features

  • Combines separate paired-end R1 and R2 files into one interleaved FASTQ.
  • Validates FASTQ structure, sequence/quality lengths, matching read identifiers, and equal read counts.
  • Supports .fastq, .fq, .fastq.gz, and .fq.gz inputs independently for each mate.
  • Preserves read identifiers with normalized /1 and /2 mate suffixes.
  • Streams inputs without loading entire datasets into memory.
  • Produces an uncompressed interleaved FASTQ for Transcript Assembly.

Statistics

Neurosnap periodically calculates runtime statistics based on job execution data. These estimates provide a general guideline for how long your job may take, but actual runtimes can vary significantly depending on factors like input size or settings used.

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API Request

Access FASTQ Interleaver using the Neurosnap API by sending a request using any programming language with HTTP support. To safely generate an API key, visit the API tab of your overview page.

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Citations

Please cite the original work when using FASTQ Interleaver in publications or research outputs.

Amani, Keaun, and Danial Gharaie Amirabadi. 2024. Neurosnap SDK Package. Software. https://github.com/NeurosnapInc/neurosnap.

Neurosnap Inc. (2022). Neurosnap: An online platform for computational biology and chemistry. Available at: https://neurosnap.ai/

Set up your run

Configure FASTQ Interleaver

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Inputs & configuration

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