PAMmla Evolve
Evolve SpCas9 PAM preference using evolutionary algorithms.
Overview
PAMmla Evolve uses an evolutionary algorithm to optimize SpCas9 PAM preference starting from random or custom amino acid sequences.
Run PAMmla Evolve on Neurosnap
The PAMmla Evolve online webserver allows anybody with a Neurosnap account to run and access PAMmla Evolve, no downloads required. Information submitted through this webserver is kept confidential and never sold to third parties as detailed by our strong Terms of Use and Privacy Policy.
Features
- Optimizes SpCas9 PAM preference using evolutionary algorithms.
- Supports random or custom starting variants.
- Allows fine-tuning of mutation rates, population size, and selection criteria.
- Can maximize or minimize affinity for specific PAMs.
- Can maximize selectivity for specific PAMs over others.
Statistics
Neurosnap periodically calculates runtime statistics based on job execution data. These estimates provide a general guideline for how long your job may take, but actual runtimes can vary significantly depending on factors like input size or settings used.
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| Credit Usage Rate | loading... |
| Estimated Total Cost | loading... |
| Runtime Mean | loading... |
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| Runtime Standard Deviation | loading... |
| Runtime 90th Percentile | loading... |
| Runtime Longest | loading... |
API Request
Access PAMmla Evolve using the Neurosnap API by sending a request using any programming language with HTTP support. To safely generate an API key, visit the API tab of your overview page.
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Citations
Please cite the original work when using PAMmla Evolve in publications or research outputs.
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Silverstein, R.A., Kim, N., Kroell, AS. et al. Custom CRISPR–Cas9 PAM variants via scalable engineering and machine learning. Nature 643, 539–550 (2025). https://doi.org/10.1038/s41586-025-09021-y |
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Neurosnap Inc. (2022). Neurosnap: An online platform for computational biology and chemistry. Available at: https://neurosnap.ai/ |