Use TIsigner Expression Optimization

The input nucleotide sequence that corresponds to a protein you want to optimize the expression of. This sequence must be divisible by 3. You can optionally also input an amino acid sequence that will then be converted into the corresponding DNA sequence.

The target expression score you wish to reach, 5 is the minimum, 30 is low, 70 is high, and 100 is the maximum.

Select the host you want to optimize protein expression in.

Select the promoter.

Translation Initiation Coding Region allows for codon substitions only in the pre-coding frame. Full-length Sequence allows nucleotide substitutions in the full sequence.

The max number of codons to replace in your sequence. If you selected 'Full-length Sequence' the limit is automatically removed.

AarI, BsaI, BsmBI and reverse complements are automatically avoided. This field must be entered as the site sequence and not the name of the enzyme, for example the SapI restriction site is GCTCTTC. Multiple restrictions sites may be entered and separated by comma.

Select the sampling method.Deep will generate 8 sequences and perform 20 iterations and Quick will generate 4 sequences and perform 10 iterations

Reports termination sequences internally for the Opening Energy calculations.

Changing this typically results in a different set of generated sequences. The default is 0 and can be set as high as 999,999,999.