Use NeuroFold

Reference / template protein structure. NeuroFold requires a template protein to bias the optimization process into generating proteins with desired properties. The reference enzyme should be as similar to the desired fully optimized enzyme as possible. The reference enzyme should at the very least be a functional enzyme. Do not worry if the the reference protein does not have optimal properties, NeuroFold will aim to optimize specified properties. Maximum length for reference is 1500 amino acids.

Specify whether you want to increase or decrease deltaG (ΔG) when compared to the reference sequence. Specifying "decrease" will create proteins that have a lower predicted ΔG compared to the reference sequence. Specifying "increase" will create proteins with a higher predicted ΔG compared to the reference sequence. A lower ΔG KCal/mol corresponds to a more stable protein structure. ΔG represents the change in free energy during the folding process of the protein. When ΔG is negative, the process is spontaneous, and the lower the value (more negative), the more favorable and stable the folded protein structure is. Conversely, a higher ΔG (less negative or positive) indicates a less stable structure, as it is less thermodynamically favorable.

Specify whether you want to increase or decrease solubility when compared to the reference sequence. Specifying "decrease" will create proteins that have a lower predicted solubility compared to the reference sequence. Specifying "increase" will create proteins with a higher predicted solubility compared to the reference sequence.

Specify the desired optimal functioning pH for the given enzyme.